KimWipes are composed of paper, silicon, and other substances. As a result of the silicon, the paper is somewhat rough feeling. KimWipes are used to wipe a variety of items, including slides and pipettes. They are often used to clean lenses as well, but lens tissue should be used instead to avoid scratching the optical surfaces. Clean the barrel of the lens.
Fold a small kimwipe into a small square pad and apply a couple of drops of methanol to a corner. Wipe down the eyeguard rubber and the barrel. Do not use kimwipes on the coated surface because the fibers of these lint-free wipes may scratch it. I like to lay down a large kimwipe to set the tools and eyepieces on while working. We had Kimwipes that were used in the process.
If you used them to blow your nose when you had a cold, you learned just how abrasive feeling those things are! You need to give me OK to edit your image and repost! I am surprised by the contents of your post Wilt. The opinions on Kimwipes seems to be all over the place! But consider the fact that they are a PAPER products producer, and paper is wood pulp, and a number of manufacturer's optics care publications allude to the fact that cotton is fine to use, but wood pulp-based products are NOT fine to use on optics.
These instructions from microscope and telescope optics manufacturers, that I have seen in past searches. If you look at the instructions from universities to its students in care and cleaning of lab optics, even that has differences of opinion from university to university! Some say to wipe optics with Kimwipes; others say to wide the exteriors of equipment and perhaps to blot up exceesses of oils for oil-immersion miscroscope optics but to use LENS TISSUE with lens cleaning fluids or very brief wiping with methyl alcohol so as to not dissolve the cements that hold the lens element in place.
Kimwipe is lint free, and relative good for wiping up spills or excess fluids, so in the university student lab setting, it makes some sense for cleaning spills. My guess is that since lots of it is around, it got conscripted for even cleaning lenses, but at some universitiesthis was done grad student lab assistants who simply did not know better, and that legacy remains with us in some places. At other universities, more astute lab assistants knew better! Lastly, I refer back to my previous comments about blowing my nose into Kimwipes in the past and how rough it was and we all know about how abraided our noses get during colds and extended use of Kleenex or other tissues based on wood pulp!
Kimberly-Clark and wood pulp products are synonymous, so would you trust your lens coatings to their products? Finally, there was a published story in Pop Photography or Modern Photography many many years ago when microfiber was not yet readily available outside of Japan, where lens tissue that was cheap off-brand items was more abrasive to coatings than Kodak Lens Tissue which was more abrasive to coatings than microfiber when the surface was viewed under high magnification.
So if the cheap 'lens tissue' abraids, dare we try Kimwipe unless we get some statement from Kimberly Clark about the freedom from wood fibers? Food for thought. Approve the Cookies This website uses cookies to improve your user experience.
By using this site, you agree to our use of cookies and our Privacy Policy. Register to forums Log in. Jan 02, 1. LIKES 0. Grey56 Grey56 Member. Jan 02, 2. Iowa, USA. Jan 02, 3. Jan 02, 4. Jan 02, 5. Tee Why wrote in post They are not very absorbant, and tend to smear the lens cleaner fluid in my view. Under what conditions would you adjust the diaphragm?
On your microscope, move the low - power objective into place. You should always begin studying a slide on low power , because this makes it easiest to find objects on the slide. Position the diaphragm so that the largest opening is used. This will allow the maximum amount of light to be used.
Parfocal means that the microscope is binocular. Parfocal means that when one objective lens is in focus, then the other objectives will also be in focus. Place it on a flat surface. Turn the coarse adjustment knob to raise the body tube. Revolve the nosepiece. Put it on Low-power objective. Adjust the diaphragm.
The bottom support of the microscope. Why should you always view a slide at lower power first? Because it is easier to locate the specimen at this power. Use the course adjustment knob to bring the speciman into focus and then use the fine adjustment knob to focus on the detail of the specimen. Since a higher magnification lens bends light more severely, the specimen is brought into focus a shorter distance from the lens and this is known as the focal length.
Generally, a lens providing higher magnification will also provide better resolution. Why should I not use the coarse focus knob for high - power objectives? Only use the fine focus knob of all others.
First, the image moves in and out of focus too quickly, so that it is difficult to precisely adjust the focus. Second, you run the risk of crashing the objective into the slide. Total magnification is when the object being viewed is magnified to its maximum limit.
Why should only lens paper be used to clean a microscope? Category: medical health eye and vision conditions. The glass on the lens is softer and will scratch easily. It allows the lenses to move independently. What is the safest solvent for cleaning an objective lens? The microscope should never be located in a position where it could be affected by acidic or alkaline vapors, such as in or near a wet chemical photographic laboratory.
In tropical regions, microscopes frequently experience a buildup of fungus. Although there are over , fungus species, two members of the genus Aspergillus are believed responsible for most lens deterioration.
Optimum growth conditions for these fungi are relatively high temperature and high humidity, but they are more adaptable to lower humidity levels than most other fungi. Fungal contamination can best be minimized by reducing the humidity of the room either by air conditioning or by installing an infrared lamp above the microscope at a minimum distance of centimeters or 5 feet. Fungi growing on glass surfaces are not attached by roots and can be wiped off, but unfortunately, residual corrosion marks remain and the original lens performance cannot be recovered and the lens must be replaced.
The only effective means to avoid fungal damage to optical components is to prevent its growth in the first place. The ideal microscopy room would be designed specifically for that purpose, and incorporate every mechanism available for limiting contamination by dust, chemical vapors, and other airborne contaminants, as well as isolating the instrument from acoustic and mechanical vibration and temperature variations.
This ideal situation is seldom realized, and most microscopes are located in areas subject to a considerable number of environmental deficiencies. Some contamination is unavoidable, due to the rigors of daily use, but at the very least, the microscope should be protected as well as possible during periods of non-use by covering the entire instrument with a suitable cover. Instrument manufacturers and aftermarket suppliers offer a variety of specially designed dust covers.
Of the several types of plastic covers available, those made of softer more flexible material are probably less prone to attraction of dust. Lint-free fabric covers are also available, and provide an effective dust barrier that can minimize the need for cleaning. While the cost of a modern research grade microscope can range from approximately a few tens of thousands to several hundred thousand dollars, if properly used and maintained, the basic optical and mechanical components of the instrument can easily outlive several generations of microscopists.
Only if the instrument is used correctly and maintained regularly is it capable of producing the best image data possible. Careless, incorrect operation and maintenance techniques not only result in unreliable and poor quality images, but cause productivity to suffer and the instrument's useful lifetime to be greatly reduced. Erin E. Wilson and Michael W.
Carl Zeiss Microscopy Online Campus. Introduction Microscopes often represent a significant investment of funds and are sophisticated optical instruments that require periodic maintenance and cleaning to guarantee successful microscopy and perfect images.
The critical areas include: The external surface of the front lens of the objective The surface of the camera sensor and its protective glass cover Both surfaces of the cover slip The surface of the microscope slide The surface of the camera adapter optics Surface of the upper lens of the condenser The outer and inner surfaces of the eyepiece as well as the upper surface of the reticule The outer surface of any protective glass covering openings through which light exits Other glass surfaces in the light path such as halogen lamps, high pressure arc-discharge lamps, fluorescence filters and beam splitters, collector lenses, or contrast and heat filters.
Carefully rotate objectives and cameras a small amount within their thread. Check the slide and cover slip by moving the specimen while focusing initially on the upper and then the lower surfaces. Check the condenser while moving it up and down and if applicable, by swiveling the front lens slightly. Long, thin wooden sticks, preferably of bamboo obtainable from Chinese restaurant suppliers or a comparable, not too flexible material High purity cotton Absorbent polyester swabs for cleaning optical components Soft cosmetic cellulose tissue specifically designed for optical surfaces Kimwipes are suitable for lens cleaning, but typical facial tissues contain hard particulates that are harmful to optical surfaces Dust blower or ear wash bulb laboratory suppliers, pharmacies Distilled water Freshly prepared solution of 5 to 10 drops of a washing-up liquid in 10 milliliters distilled water Solvent for the removal of greasy or oily dirt, such as the Optical Cleaning Solution L recipe of 85 percent petroleum ether, 15 percent isopropanol , pure petroleum ether analytically pure, boiling point less than 44 degrees Celsius or, exclusively for cleaning cover slips, pure acetone.
Wash hands powdered, latex gloves are not suitable. Dip the stick into the cleaning solution aqueous or organic solvent. The cotton fibers attach better to the stick as a result. Dab the stick onto the wad of percent cotton and loosen some fibers.
Do not compact the cotton otherwise the fibers will not separate easily. Turn the stick so that an even, elliptical cotton bud forms at the tip. To protect the cotton tip from dirt, the stick should be stored in a plastic sandwich bag. It should not be handled as perspiration and grease from fingers will significantly affect its ability to clean. Remove the cotton tip after every wipe and replace it with a fresh cotton bud.
The stick can be used for a long period of time. Use separate sticks for water-based solutions and organic solvents. If the use of a high purity cotton lens cleaning tissue is preferred, fold the sheet around the stick so that a sharp point is generated. The point should not be handled. Use the tissue only once and then replace it. Polyester swabs can be used until they no longer clean well.
Place the objectives, eyepieces, and cameras on a dust-free surface fresh aluminum foil, for example. Before any cleaning is attempted, the optical surface should be inspected with magnification under reflected light to determine the condition of the component.
Particular attention should be given to the presence of any particulate material, which must be assumed to be abrasive, and removed before any other cleaning is done. A magnifying lens of 2x to 3x such as a loupe is appropriate for examining larger optics such as oculars and condensers, while the smaller lens elements of objectives require approximately 5x to 10x magnification for proper inspection.
It is crucial that particulate matter be removed from a lens surface as the first step in cleaning, because any particle can be abrasive and result in scratches if it is brushed across the surface with even the most gentle lens tissue.
All other optical components to be cleaned should be as accessible as possible. Blow all loose dust particles away with a compressed gas duster, air blower, or a clean camelhair brush. Never blow dust off lens surfaces with a strong breath , and make sure to gently blow air across not perpendicular to the lens surface. The safest method of air dusting is to use a rubber bulb or balloon, such as models intended for use as ear and enema syringes for infants.
Care is required to avoid touching the tip of the syringe to the lens surface. The best advice is to avoid any use of compressed air cans for lens cleaning. It is difficult with these to control the pressure of air impinging on the surface being cleaned, and there is always the risk of either extremely cold air or freezing liquid being expelled onto a lens surface and causing irreparable damage.
Remove all water-soluble dirt with distilled water. If this step is unsuccessful, repeat using a solution of diluted washing-up liquid. Remove any remaining residue with a dry cotton swab, but breathe with an open mouth on the surface first to generate a film of moisture. In so doing, be careful to not spray droplets of saliva. An effective method of preparing lens paper for this cleaning method is to fold all four corners of a piece of tissue together, leaving the untouched center of the tissue bulging out.
The corners can be twisted together slightly to form a stem for handling the tissue. When the tissue is held by this stem, and wiping performed with the puffed-out tissue center, the force that can be applied to the objective is limited by the springiness of the tissue. Circular wiping motion can be applied in this manner, with very little direct force on the lens surface. To remove oily dirt, use a solution of dilute washing-up liquid initially.
If this action does not produce a satisfactory result, repeat the cleaning using a solvent Optical Cleaning Solution L or petroleum ether. Greasy dirt must always be removed using a solvent. Dip the cotton or polyester swab into the cleaning solution and shake off excess liquid. An excess of liquid in a cotton bud will flow over the rim of the lens and attack the lens cement. This may consequently lead to the removal of the cement between bonded components.
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